AUTOPHAGOSOME CONTENT PROFILING USING PROXIMITY BIOTINYLATION PROTEOMICS COUPLED TO PROTEASE DIGESTION IN MAMMALIAN CELLS

Autophagosome content profiling using proximity biotinylation proteomics coupled to protease digestion in mammalian cells

Summary: The ascorbate peroxidase APEX2 is commonly used to Book study the neighborhood of a protein of interest by proximity-dependent biotinylation.Here, we describe a protocol for sample processing compatible with immunoblotting and mass spectrometry, suitable to specifically map the content of autophagosomes and potentially other short-lived en

read more

Collective Memories of WWII Collaboration in Belgium and Attitudes About Amnesty in the Two Main Linguistic Communities

Collaboration with the Nazi occupier during WWII has always been a topic of dissent Oven Globe between French-speakers (FS) and Dutch-speakers (DS) in Belgium.According to a popular myth coined after the war and often narrated in the media and literature, collaboration was widespread in Flanders, whereas Walloons bravely resisted, although historic

read more

A purely mechanical model with asymmetric features for early morphogenesis of rod-shaped bacteria micro-colony

To model the morphogenesis of rod-shaped bacterial micro-colony, several individual-based models have been proposed in the biophysical literature.When studying the shape of micro-colonies, most models present interaction forces such as attraction or filial link.In this article, we propose a model where the bacteria interact only through non-overlap

read more